Figure 6. Human, but not mouse KSper is sensitive to the Slo1 channel blocker iberiotoxin (IbTX).
(A) Representative human IKSper traces under control conditions (black) and in the presence of 100 nM IbTX (red) elicited in response to the shown voltage ramp. (B) Mean current densities (CDs) ± SEM calculated at +120 mV. CDs (human) were 74 ± 8 pA/pF (control), 9 ± 1 pA/pF (IbTX), and 70 ± 19 pA/pF (washout). (C) Representative mouse IKSper traces under control conditions (black) and in the presence of 100 nM IbTX (red) elicited in response to the voltage ramp as in (A). (D) CDs (mouse) were 244 ± 11 pA/pF (control) and 235 ± 4 pA/pF (IbTX). (n), number of experiments. Four human and three mouse sperm cells were used.