Figure 7. Human, but not mouse KSper is sensitive to the Slo1 channel blocker paxilline (Pax).
(A) Representative human IKSper traces under control conditions and in the presence of paxilline elicited in response to the indicated voltage ramp. (B) Mean current densities (CDs) ± SEM calculated at +120 mV. Cells from three donors were used. CDs (human) were: 53 ± 4 pA (control), 20 ± 2 pA (100 nM paxilline), and 69 ± 6 pA (washout). (C) Representative mouse IKSper traces under control conditions and in the presence of paxilline elicited in response to the voltage ramp as in (A). (D) CDs (mouse) were: 119 ± 5 pA/pF (control) and 113 ± 10 pA/pF (500 nM paxilline). (n), number of experiments. Four human and three mouse sperm cells were used.