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Experiment C. Mean ± SD (n = 6). Measured concentrations of heroin, 6-MAM, and morphine in plasma and brain from rats infused over the course of 1 min with 0.52 mg/kg heroin. Rats were sacrificed 1 min after infusion. Samples were collected with 4 mg/mL NaF, diluted with formate buffer containing 4 mg/mL NaF, and analyzed immediately after extraction.
