Table 1.
Summary of stability experiments.
| Experiment | Matrix | NaF | Storage temp. | Duration | Figure |
|---|---|---|---|---|---|
| A (whole blood versus plasma) | Rat whole blood | Collected with NaF | 4°C | Processed immediately | 3 |
| Rat plasma | 4 mg/mL | ||||
| B (centrifuge speeds) | Rat whole blood | Collected with NaF | 4°C | Processed immediately | Not shown |
| C (live rat infusion) | Rat plasma | Collected with NaF | 4°C | Processed immediately | 4 |
| Rat brain homogenate (in formate) | |||||
| D (NaF comparison) | Rat plasma | 4 mg/mL NaF added in half samples | 4°C | 10:00 min | 5 |
| E (heroin degradation in brain) | Rat brain homogenate (in water) | 4 mg/mL | 4°C | 30:00 min | 6 |
| Rat brain homogenate (in water) | |||||
| Rat brain homogenate (in formate) | 4 mg/mL | ||||
| Rat brain homogenate (in formate) | |||||
| F (50:50 storage) | Rat dosed with heroin | 4 mg/mL in formate and plasma | −80°C | 24 h | 7, 8 |
| Rat plasma | 4 mg/mL in formate and plasma | ||||
| Rat plasma | 4 mg/mL in plasma | ||||
| Rat brain homogenate (in formate) | 4 mg/mL | Not shown | |||
| G (dried pellet) | Rat dosed with heroin | 4 mg/mL in formate and plasma | −80°C | 7 days | 8 |
| Human plasma | 4 mg/mL | Not shown | |||
| Bovine serum | 4 mg/mL | ||||
| Rat plasma | 4 mg/mL |