Table 3.

Kinetic parameters for purified extracts of the PAL enzyme from Trichosporon cutaneum.

Trial	Phenylalanine	Phenylalanine	Phenylalanine	Tyrosine	Tyrosine	Tyrosine	PAL/TAL ratio
	V max⁡ (μM min−1)	K m (mM)	V max⁡/K m	V max⁡ (μM min−1)	K′ (mM)	V max⁡/K′
1	1.3 ± 0.1	5.0 ± 1.1	0.3	0.2 ± 0.02	2.4 ± 0.6	0.1	3.0
2	4.7 ± 0.6	5.7 ± 3.0	0.8	1.0 ± 0.1	2.0 ± 0.4	0.5	1.6
3	7.2 ± 0.8	4.2 ± 2.0	1.7	0.8 ± 0.1	0.9 ± 0.4	0.9	1.9

For each trial, activity assays for Phe and Tyr were performed on the same enzyme extract. Extracts were obtained from anion exchange chromatography.