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. 2013 Sep 16;2013:205316. doi: 10.1155/2013/205316

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Copyright © 2013 Josie Resende Torres da Silva et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Time course of the changes induced by intrathecal neostigmine (neo) or saline (10 μL) on antinociception induced by sham (open symbols) or real (black symbols) electroacupuncture (EA) at 2 (a), 100 (b), or 2/100 (c) Hz frequency on the tail-flick latency of rats anesthetized with isoflurane. Anesthesia was performed during the period indicated by a horizontal dashed arrow. Neostigmine (0.1 or 0.2 μg/10 μL) was injected at the time indicated by a vertical arrow. EA was applied for 20 minutes (horizontal line). Points are means (± SD) of 6 rats per group. P < .05 compared to saline/sham EA (∗) or any other group (#) using the Bonferroni post hoc test.