Figure 5.

Allosuppressive potential of single-MSC derived clones and non-cloned MSCs. (A). The allosuppressive effect of clones derived from CD271-MSC and PA-MSCs which have been generated from 2 bone marrow donors. Triangles represent the MSC-clones of the first donor, whereas circles represent MSC-clones from the second donor. (B) CD271-MSC derived clones, which whose allosuppressive effects (red bars) are completely mediated by PGE2, because treatment with indomethacin abrogated completely this effect (green bars). (C) CD271-MSC derived clones which use partially PGE2 as a mediator of allosuppressive activity (red bars), while treatment with indomethacin can only partially reverse the inhibitory effect on proliferation of MNCs (green bars). (D) CD271-MSC derived clones, which are PGE2-independent, because the treatment with indomethacin as an inhibitor of PGE2 synthesis (green bars), was not able to abrogate the inhibitory potential of clones on proliferation of the mononuclear cells in the presence of MSC-clones (red bars).