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. Author manuscript; available in PMC: 2014 Sep 13.
Published in final edited form as: Biochem Biophys Res Commun. 2013 Aug 21;439(1):6–11. doi: 10.1016/j.bbrc.2013.08.042

Figure 3.

Figure 3

miR-143 decreases COX-2 mRNA stability and inhibits p-MEK/p-MAPK/RREB1 and COX-2

(A) BxPC-3 and (B) HPAF-II transfected with miR-143 or control RNA and dosed with 1 μM of actinomycin D were screened for COX-2 and GAPDH mRNA expression. Ct values standardized to GAPDH are represented as mean fold expression ± SEM with statistical significance at p<0.05 as indicated by the asterisk (*). (C) BxPC-3 and HPAF-II transfected with 100 nM of miR-143 were Western blotted for p-p38MAPK, p-MEK, p-MAPK, RREB1, COX-2 and GAPDH. Protein bands visualized by ECL were quantified using digital imaging software.