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. 2013 Oct 3;8(10):e76981. doi: 10.1371/journal.pone.0076981

Figure 3. D(KLAKLAK)2 induces ultrastructural cellular changes in R. oryzae.

Figure 3

TEM of germlings (6,000-fold) incubated with D(KLAKLAK)2 (150 µg/ml, 300 µg/ml) revealed extensive vacuolization comparable to that induced by AMB (2 µg/ml). The increase in number and size of vacuoles was visualized with the vacuolar probe FM4-64 (red). staining of intact plasma membrane FM4-64 diffusion into the cytoplasm and staining of fragmented vacuoles in treated D(KLAKLAK)2 (150 µg/ml, 300 µg/ml) and AMB (2 µg/ml). MitoTracker staining (green) indicated morphological changes and swelling of mitochondria in D(KLAKLAK)2-exposed germlings as opposed to the punctate pattern observed in samples treated with control drugs. TEM scale bar, 2 µm. Fluorescent micrographs scale bar, 200 µm.