Figure 1. CaP60A ^kum170 mutant has altered heart rate and cardiac dimensions after heat shock.

A. Percent paralysis after heat shock. CaP60A ^kum170 heterozygote flies were exposed to heat shock of varying durations (no heat shock defined as 0 min (closed circle), 5 min (closed triangles), 7 min (open triangles), or 10 min (open circles)) and observed for up to 72 hours. All flies receiving a heat shock of greater than 7 minutes developed irreversible paralysis; while a smaller percentage of flies developed paralysis after 5-minute heat shock. B. Representative optical coherence tomography (OCT) recordings from w ¹¹¹⁸ and CaP60A ^kum170 after varying durations of heat shock (HS). End diastolic dimension (EDD) and end systolic dimension (ESD) are denoted in red; A 125 micron standard and one-second bar are shown. C. Heart rates measured from 3 second OCT recordings show a progressive decline in heart rate with increasing durations of heat shock. D. End diastolic dimensions (EDDs) increase with increasing duration of heat shock. E. Fractional shortening is not markedly altered with heat shock in the CaP60A^kum170 mutants. *p<0.05, † p<0.005, ‡p<0.0001 in comparison to 0 minutes heat shock by one-way ANOVA with Tukey’s multiple comparisons test. N= 16, 12, 16, 4, 22 for 0, 5, 6, 7 and 10 minute groups, respectively.