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. 2013 Oct 3;8(10):e74596. doi: 10.1371/journal.pone.0074596

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© 2013 Han et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A: The mRNA levels of apoptosis related genes were examined by q-PCR. The results demonstrated that Caspase-3 and Bcl-2 was significantly decreased in pshRNA2 group 48 h post-transfection [p<0.05, compared with pshRNA-negative and PBS groups] but the levels of Bax and p53 gene was unlatered [p>0.05]. B: The protein levels of apoptosis related genes were detected by western blotting. The results showed that Caspase-3, Bax and Bcl-2 genes was up-regulated by inhibin gene silencing. But p53 gene was not affected by inhibin silencing.