Figure 6. Immuno-gold labeling and electron microscopic analysis of LL-37 binding to OMVs.

The following samples were analyzed: a) Control-OMVs from A1552 grown without AMPs, b) OMVs from A1552 grown with PmB, c) OMVs from the Δbap1 mutant grown without PmB), d) OMVs from the Δbap1 mutant grown with PmB, e) OMVs from the ΔompT mutant grown without PmB, f) OMVs from the ΔompT mutant grown with PmB. Arrows in panel b) indicate examples of gold labeling. Horizontal bars represent 100 nm.