Table 2. Protein identifications in A. suum L3-lung ES products.
Category/Protein identitya | IDb | Score | Coverage | Unique sequences | emPAI | Sigc |
% | ||||||
Metabolic pathway | ||||||
Maltase-glucoamylase | GS_07553 | 78 | 2 | 1 | 0.05 | + |
GS_15893 | 142 | 6 | 2 | 0.15 | − | |
GS_16769 | 132 | 8 | 3 | 0.18 | ++ | |
GS_23879 | 171 | 4 | 4 | 0.08 | ++ | |
Neprilysin-1 | GS_08219 | 66 | 1 | 1 | 0.02 | + |
Sucrase-isomaltase | GS_01568 | 277 | 19 | 4 | 0.66 | − |
GS_02444 | 258 | 13 | 3 | 0.28 | − | |
GS_05716 | 80 | 11 | 1 | 0.19 | − | |
GS_08447 | 69 | 2 | 1 | 0.04 | ++ | |
GS_17323 | 129 | 3 | 1 | 0.05 | − | |
GS_20796 | 121 | 2 | 1 | 0.04 | − | |
GS_22047 | 151 | 6 | 3 | 0.12 | ++ | |
Structural | ||||||
Cuticlin-1 | GS_10816 | 63 | 3 | 1 | 0.09 | − |
Cuticle collagen 12 | GS_16238 | 147 | 17 | 2 | 0.27 | ++ |
Cuticle collagen 13 | GS_12737 | 289 | 8 | 1 | 0.34 | ++ |
Peptidyl-prolyl cis-trans isomerase B | GS_15602 | 197 | 7 | 2 | 0.29 | − |
Binding | ||||||
14-3-3-like protein | GS_05590 | 73 | 3 | 1 | 0.11 | − |
C-type lectin | GS_12842 | 102 | 49 | 2 | 1.70 | − |
Latent-transforming growth factor beta-binding protein 1 | GS_21305 | 102 | 0 | 1 | 0.01 | ++ |
Thyrotropin-releasing hormone-degrading ectoenzyme | GS_02555 | 123 | 4 | 1 | 0.08 | ++ |
Transmembrane cell adhesion receptor mua-3 | GS_11192 | 253 | 2 | 6 | 0.06 | ++ |
Other | ||||||
Aspartic protease 6 | GS_13572 | 239 | 9 | 2 | 0.25 | + |
Pepsin inhibitor Dit33 | GS_22518 | 81 | 6 | 1 | 0.13 | ++ |
Poly(U)-specific endoribonuclease | GS_22743 | 101 | 4 | 1 | 0.05 | + |
Protein DAO-2 | GS_24324 | 120 | 13 | 1 | 0.54 | + |
Serine protease | GS_07735 | 78 | 2 | 1 | 0.05 | + |
Serpin-like protein | GS_19115 | 303 | 14 | 2 | 0.29 | − |
Unknown | ||||||
24 kDa protein of As22 | GS_08591 | 219 | 17 | 2 | 0.36 | + |
DOMON domain-containing protein | GS_00339 | 144 | 20 | 2 | 0.43 | − |
Excretory/secretory mucin MUC-5 | GS_22776 | 529 | 56 | 1 | 4.62 | ++ |
Heh-1 | GS_20415 | 66 | 5 | 1 | 0.12 | − |
Transthyretin-like protein 5 | GS_01881 | 85 | 9 | 1 | 0.25 | + |
Venom allergen 3 | GS_10381 | 103 | 12 | 2 | 0.26 | ++ |
von Willebrand factor domain-containing protein | GS_02090 | 66 | 3 | 1 | 0.07 | ++ |
Hypothetical protein LOAG_00319 | GS_14306 | 72 | 14 | 1 | 0.30 | − |
Hypothetical protein LOAG_07538 | GS_11367 | 109 | 5 | 1 | 0.12 | − |
Unknown | GS_01811 | 72 | 14 | 1 | 0.29 | − |
Unknown | GS_02698 | 83 | 11 | 1 | 0.33 | − |
Unknown | GS_03310 | 89 | 4 | 1 | 0.14 | + |
Unknown | GS_09456 | 119 | 20 | 2 | 0.56 | + |
Unknown | GS_10718 | 171 | 34 | 2 | 0.92 | + |
Unknown | GS_12589 | 133 | 11 | 1 | 0.58 | + |
Unknown | GS_15853 | 245 | 18 | 2 | 0.67 | − |
Unknown | GS_17230 | 198 | 11 | 2 | 0.49 | − |
Unknown | L3E_00366 | 150 | 4 | 1 | 0.21 | − |
The proteins identified were categorized by their molecular function according to information obtained from the Gene Ontology database.
The accession number in A. suum genome database (available on WormBase, www.wormbase.org).
The identified proteins were predicted to be either a classical secretory protein (+), non-classical secretory protein (++) or not secreted (−) by secretion prediction using SignalP and SecretomeP.