Figure 1.

N-ethyl-N-nitrosourea (ENU) breeding scheme and visible morphology of skeletal morphology mutants (Skm). (A) Schematic representation of the breeding scheme used during the identification of deviant pedigrees. 129S1 male G0 mutagenized mice were crossed to 129X1 females and the resulting G1 males were outcrossed to DBA/2J females to generate G2 progeny. Visible skeletal malformations in Skm1 (B) mice and Skm2 (C) mice. (B) Skm1 mice show kyphotic spines and kinked tails (left) and elongated bodies (right). (C) Skm2 mice exhibit severe scoliosis and lordosis.