Figure 3.

Map-based cloning of ASL1: (A) The ASL1 locus initially was mapped to a region between markers RM488 and RM297 on the long arm of rice chromosome 1 (Chr1) with 190 recessive individuals. (B) Fine mapping of ASL1 to BAC2 (AP003335) within a 60-kb region by the markers P4 and P6 using 3478 recessive individuals. (C) Diagram of the predicted ORFs and the mutation site. A 2-bp deletion (GA) in ORF8 results in a premature stop codon; (D) Gene model of ASL1. (E) Complementation of the asl1 mutant. The asl1 mutants transformed with pCAMBIA1301-ASL1 vector (left) show normal green leaves, whereas the mutants transformed with empty pCAMBIA1301 vector (right) have albino leaves. (F) T₁ of transgenic plants.