Figure 2.

Hermaphrodite self-reproduction antagonizes mating. (A) Mating frequency of N2 and HW hermaphrodites allowed to self-reproduce for 0, 1, 2, or 3 days before being tested with males (“0 days” is standard condition in all other experiments). (B) Reproductive tempo (i.e., self-progeny and self-sperm depletion) of isolated hermaphrodites in the period after the same treatment in (A). (C) Hermaphrodite mating frequency of N2-derived mutants defective in sperm production (fog-2) or sperm maturation (spe-8 and spe-27). (D) Mating frequency of all paired combinations of N2, HW, and fog-2(q71) hermaphrodites and males. Unless otherwise noted, HW males were used as the tester strain to dissect hermaphrodite mating frequency. Bar graphs depict mean ± SEM of multiple trials. *P < 0.05 and ⁺⁺P < 0.01 by permutation test stratified by trial. n.s., not significant. **Significant comparisons between hermaphrodite genotypes (pooling male genotypes) and ⁺⁺ between male genotypes (within each hermaphrodite genotype).