Table 1. S. japonicus strains genotype.
| Strain | Iod | Genotype | Source |
|---|---|---|---|
| Sjk2 | + | mat1-P, mat2/3Δ | Yu et al. (2013) |
| Sjk3 | + | mat1-M, mat2/3Δ | Yu et al. (2013) |
| Sjk10 | + | mat1-P, mat2/3Δ, ade6-dam-E, ura4-D3 | This study |
| Sjk17 | + | h+, mat-2017, mrc1Δ::Nat | This study |
| Sjk19 | + | mat1-M, mat2/3Δ, mrc1Δ::Nat | This study |
| NIG6701 | - | h+, mat-2017, mrc1Δ::Nat | Furuya and Niki (2012) |
Iod indicates iodine vapor−staining colony phenotype when cells of opposite mating type are mated for meiosis and sporulation. The symbol Δ indicated deletion of the gene. The mat2/3Δ donor loci−deleted strains were derived from Sjk4, which is homothallic for the mating-type switching phenomenon (Yu et al. 2013). Nat is a Nourseothricin gene cassette that replaced the mrc1 gene in the genome; it confers the Nourseothricin antibiotic resistance to yeast. The ade6-damE and ura4-D3 markers were derived from a stock provided by Dr. H. Niki.