Figure 2. Morphological characterization of the spoIIID and spoVT mutants.

Cells of the C. difficile 630Δerm strain, the spoIIID or spoVT mutant and the complemented strains, CDIP262, carrying a multicopy spoIIID gene controlled by its native promoter or CDIP263, carrying a multicopy spoVT gene controlled by its native promoter were collected after 24 h of growth in SM broth, stained with the DNA stain DAPI and the membrane dye FM4-64 and examined by phase contrast and fluorescence microscopy.