Table 3. Control of σG or σK target genes by both σF and σE.
Gene | Function | Expression ratio | |||
Forespore σG-dependent | sigF/630Δerm | sigE/630Δerm | sigG/630Δerm | ||
transcriptome | |||||
CD1486 | Putative ribosome recycling factor | 0.10 | 0.26 | 0.17 | |
CD1631 | sodA | Superoxide dismutase (Mn) | 0.06 | 0.21 | 0.32 |
CD1880 | Conserved hypothetical protein | 0.07 | 0.21 | 0.25 | |
CD3249 | sspB | Small, acid-soluble spore protein beta | 0.01 | 0.04 | 0.07 |
CD3551.1 | Putative membrane protein | 0.11 | 0.31 | 0.18 | |
qRT-PCR | |||||
CD0773 | spoVAC | DPA uptake protein, SpoVAC | <0.01 | 0.06 | 0.02 |
CD0792 | Putative membrane protein, DUF81 family | <0.01 | 0.02 | 0.01 | |
CD1213 | Stage IVB sporulation protein B, peptidase | 0.04 | 0.08 | 0.12 | |
CD1290 | Putative small acid-soluble spore protein | <0.01 | 0.05 | 0.01 | |
CD1430 | pdaA | Putative d-lactam-biosynthetic deacteylase | <0.01 | 0.14 | 0.03 |
CD2375 | Conserved hypothetical protein | 0.03 | 0.24 | 0.05 | |
CD2465 | Putative amino acid/polyamine transporter | 0.18 | 0.15 | 0.19 | |
CD2636 | Putative membrane protein | <0.01 | 0.13 | 0.03 | |
CD2688 | sspA | Small, acid-soluble spore protein alpha | <0.01 | 0.05 | 0.02 |
CD3499 | spoVT | Transcriptional regulator, SpoVT | <0.01 | 0.06 | 0.07 |
A gene is considered differentially expressed when the P value is <0.05 using the statistical analysis described in Materials and Methods.
qRT-PCR were performed as described in Materials and Methods.