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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Nat Cell Biol. 2013 Aug 25;15(10):1153–1163. doi: 10.1038/ncb2827

Figure 8.

Figure 8

miR-205 is required to maintain pAkt levels by targeting multiple negative regulators in skin progenitors and SCs. (a) pAkt levels are significantly decreased in miR-205 KO epidermis (Epi), HFSCs and ORS, but only slightly decreased in matrix (Mx). (b) pAkt is significantly downregulated in Dicer cKO (Dcr cKO) epidermis (Epi). (c) Keratinocytes infected with miR-205 targets (Inpp4b, Frk, Phlda3 and Inppl1) show lower pAkt levels compared to control (MSCV) infected cells. (d) Forced expression of individual miR-205 targets compromises the capacity of primary keratinocytes to form productive colonies. Data shown are mean ± s.d. from five independent experiments. *, P < 0.05; **, P < 0.01; ***, P< 0.001 by Student’s t-test. (e) Constitutively active Akt (myr-Akt) rescues the growth defects of miR-205 KO HFSCs in vitro. Data shown are mean ± s.d. from five independent experiments. ***, P < 0.001 by Student’s t-test. (f) A model illustrates the role of miR-205 in the proliferation and expansion of skin progenitors and SCs by antagonizing multiple negative regulators of the PI3K/Akt pathway. Uncropped images of blots are shown in Supplementary Fig. S9.