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. 2013 Sep 18;33(38):15132–15144. doi: 10.1523/JNEUROSCI.4748-12.2013

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Copyright © 2013 the authors 0270-6474/13/3315132-13$15.00/0

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Figure 5. — Expression of Tgf-β-members in sympathetic ganglia at different developmental stages. cDNA from E18.5, P3, and P90 sympathetic ganglia was analyzed for the expression of several TGF-β-members. Bmps -2, -3, -4, -5, -7, and Gdf1 are mainly expressed at early developmental stages (E18.5 and P3). At P90 only Bmp-2, Bmp-4, and Bmp-6 are expressed at low levels. Gdf11 is not expressed in sympathetic ganglia. In the Tgfβ-group Tgf-beta1 shows similar expression patterns as Bmps, Tgf-beta2 is only strongly expressed at E18.5, and Tgf-beta3 is barely detectable in sympathetic ganglia. Activin A and Alk2 are detectable throughout development up to adult stages. RT-PCR reactions for Bmp-6, Bmp-7, and Tgf-beta1–3 and their corresponding Gapdh reactions were analyzed on different gels and the bands assorted into Bmp-6/7 and TGF-beta1-3 subfigures.