Fig. 5.
Effects of CSE, CARB and FP on the necrosis and apoptosis of bronchial epithelial cells (16-HBE). 16-HBE cells were cultured in the presence and absence of CSE (5 and 10 %), CARB (10−4 M) and FP (10−8 M) for 18 h and then were used for assessing cell necrosis and cell apoptosis by the annexin V/PI method using flow cytometry (see “Materials and methods” for details). Representative dot plots showing the percentage of annexin V, PI and annexin V/PI-positive 16-HBE at baseline and following the exposure of CSE (5 and 10 %), CARB (10−4 M) and FP (10−8 M) are shown