Fig. 7.
Effects of CARB and FP on HO-1 expression in bronchial epithelial cells. 16-HBE (n = 5) cells were cultured in the presence and absence of CSE (10 %), CARB (10−4 M) and FP (10−8 M) for 18 h. Total proteins were extracted and analysed for HO-1 expression using western blot analysis. Membranes were then stripped and incubated with rabbit polyclonal anti-ß-actin. a Densitometric analysis of HO-1 expression. Signals corresponding to HO-1 on the various western blots were semi-quantified by densitometric scanning, normalised and expressed after correction with the density of the band obtained for beta-actin. Data are expressed as arbitrary units ± SD. b Representative western blot performed on nuclear extracts. Lane 1, baseline; lane 2, CSE 10 %; lane 3, CARB (10−4 M); lane 4, FP (10−8 M); lane 5, CSE 10 % + CARB (10−4 M); lane 6, CSE 10 % + FP (10−8 M)