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. 2013 May 2;18(6):773–783. doi: 10.1007/s12192-013-0429-8

Table 1.

Gene special primers used in the real-time quantitative reverse transcription PCR

Gene Serial number Primer sequence Primer length (bp) Size of the products (bp)
Hsp90 NM_001109785.1 Forward 5′-TCCTGTCCTGGCTTTAGTTT-3′ 20 143
Reverse 5′-AGGTGGCATCTCCTCGGT-3′ 18
Hsp70 NM_001006685.1 Forward 5′-CGGGCAAGTTTGACCTAA-3′ 18 250
Reverse 5′-TTGGCTCCCACCCTATCTCT-3′ 20
Hsp60 NM_001012916.1 Forward 5′-AGCCAAAGGGCAGAAATG-3′ 18 208
Reverse 5′-TACAGCAACAACCTGAAGACC-3′ 21
Hsp40 NM_001199325.1 Forward 5′-GGGCATTCAACAGCATAGA-3′ 19 151
Reverse 5′-TTCACATCCCCAAGTTTAGG-3′ 20
Hsp27 NM_205290.1 Forward 5′-ACACGAGGAGAAACAGGATGAG-3′ 22 158
Reverse 5′-ACTGGATGGCTGGCTTGG-3′ 18
COX-2 NM_001167718 Forward 5′-TGTCCTTTCACTGCTTTCCAT-3′ 21 84
Reverse 5′-TTCCATTGCTGTGTTTGAGGT-3′ 21
iNOS NM_204961 Forward 5′-CCTGGAGGTCCTGGAAGAGT-3′ 20 82
Reverse 5′-CCTGGGTTTCAGAAGTGGC-3′ 19
PTGEs NM_001194983 Forward 5′-GTTCCTGTCATTCGCCTTCTAC-3′ 22 115
Reverse 5′-CGCATCCTCTGGGTTAGCA-3′ 19
TNF-α NM_204267 Forward 5′-GCCCTTCCTGTAACCAGATG-3′ 20 82
Reverse 5′-ACACGACAGCCAAGTCAACG-3′ 20
NF-κB NM_205134 Forward 5′-TCAACGCAGGACCTAAAGACAT-3′ 22 162
Reverse 5′-GCAGATAGCCAAGTTCAGGATG-3′ 22
β-actin L08165 Forward 5′-CCGCTCTATGAAGGCTACGC-3′ 20 128
Reverse 5′-CTCTCGGCTGTGGTGGTGAA-3′ 20