Fig. 2.

Tα-syn is taken up by OB cells less than 20 min after injection in the OB. Huα-syn- (stained with syn211 antibody) and BSA-staining in the OB, 20 min after injection of tα-syn, tBSA or ATTO-550 into the OB: a low magnification pictures of the ipsilateral OB (GL/Mit/GCL) (scale bar 50 μm), or high magnification pictures (scale bar 10 μm) of glomerular layer (GL) (b), mitral layer (Mit) (c), and granular cell layer (GCL) (d) of the ipsilateral OB. At 20 min after injection of monomeric, oligomeric and fibrillar tα-syn into the OB, we detected huα-syn-positive cells in various layers of the OB (GL, Mit, and GCL), but no BSA-positive cells in the OB injected with tBSA. A diffuse BSA-staining was observed in extracellular space. To control for specificity of our immunohistochemistry protocol, sections from groups that were injected with ATTO-550 into the OB were stained for huα-syn, and did not reveal any huα-syn-positive staining. A white arrow points examples of huα-syn-positive cells. e Uptake of injected α-syn at 20 min: number of huα-syn positive mitral cells in the ipsilateral OB at 20 min (H = 6.053, P < 0.04, post hoc test: monomers/fibrils P < 0.05). Scatter plots show data from individual mice