FIGURE 4.

ClC-7/Ostm1 activation kinetics depend on the length of the linker between the transmembrane domain and CBS1. A, diagram showing the sequence of the linker between the last intramembrane helix R and CBS1. Deletion and insertion constructs are shown. B, typical two-electrode voltage clamp traces obtained with these constructs upon co-expression with Ostm1. C, evaluation of corresponding activation (τ_act) and deactivation (τ_deact) time constants. The voltage clamp protocol is as in Fig. 1. Thick lines indicate arithmetic mean, and thin lines indicate ± S.E.