TABLE 2.
VKOR activity supported by VKORC1L1 enzyme in different tissues of male OFA Sprague-Dawley rats
Percentages of the VKOR activity catalyzed by VKORC1 or VKORC1L1 were evaluated by inhibition of the VKOR activity by warfarin at varying concentrations of vit K1>O and by modeling of the experimental results by nonlinear regression using the noncompetitive inhibition equation, the respective Km and Ki values determined for recombinant VKORC1 and VKORC1L1 proteins and considering a mixed expression of VKORC1 and VKORC1L1 in the tissue.
| VKOR activity | Liver | Lung | Kidney | Brain | Testis | ROS 17/2.8 |
|---|---|---|---|---|---|---|
| % | % | % | % | % | % | |
| Supported by VKORC1L1 | 4 | 22 | 1 | 9 | 55 | 69 |
| Supported by VKORC1 | 96 | 78 | 99 | 91 | 45 | 32 |