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Effect of added mtHSP70 on selected peaks from 1H,15N TROSY-HSQC spectra of U-15N-ISCU and U-15N-ISCU(N90A). A, U-15N-ISCU alone (red) and U-15N-ISCU with 0.2 eq of added unlabeled mtHSP70 (blue). B, U-15N-ISCU(N90A) alone (red) and U-15N-ISCU(N90A) with 0.2 eq of added unlabeled mtHSP70 (blue).
