FIGURE 2.

Heparin binding characteristics of AAV2, AAV2G9, and AAV9. A, heparin affinity column chromatogram of parental AAV2 vectors. A total of 1e11 vg containing particles of AAV2-CBA-Luc in 1 ml of 1× PBS was loaded onto a heparin affinity column. Bound virions were eluted from the heparin affinity column by elution with increasing NaCl concentration (right y axes, gray lines). The numbers of vg in the loaded sample and each eluted fraction were quantified using qPCR as described under “Experimental Procedures.” The numbers of vg in each fraction were normalized to the total loaded vg to obtain the percentage of recovered virions (left y axes, black lines). Heparin affinity column chromatograms of AAV2G9 (B) and AAV9 (C) were obtained through a similar approach.