FIGURE 6.

Competitive inhibition of AAV2G9 and AAV2i8G9 by parental AAV serotypes. A, CHO Lec2 cells were preincubated with AAV2 or AAV9-CBA-tdTomato at MOI ranging from 500–100,000 vg/cell for 2 h. Pretreated cells were then infected with AAV2G9-CBA-Luc particles (MOI = 1000 vg/cell). At 24 h post-transduction, cells were lysed to assess luciferase activity. Percentage inhibition of AAV2G9 transduction was calculated by normalizing luciferase transgene expression levels to that of control with no AAV competitors. B, competitive inhibition of AAV2i8G9-CBA-Luc transduction by AAV2i8-tdTomato and AAV9-tdTomato was carried out in a similar fashion. The percentage of luciferase expression was obtained by normalizing the luciferase transgene expression in each condition by that of the control condition without any competitor AAV. Results are presented as mean ± S.E. (n = 4). Statistical significance was analyzed using one-tailed Student's t test. *, p < 0.05; **, p < 0.01.