FIGURE 4.

Absence of MAGP2Δ9 mRNA confirms functional knockout of MAGP2. A, polyadenylated mRNA from the heart, lung, and kidney of two DKO, 2KO, and WT animals each was reverse-transcribed and subjected to 27 rounds of PCR amplification with primers specific for the Mfap5 message. Arrows indicate WT and 2Δ9 amplicons (2KO). Reactions with primers to β-actin were run in tandem as a loading control. B, PCR genotyping of genomic DNA from animals used in the assay. WT, 2KO, and 1KO amplicons are indicated by arrows. NoRT, no reverse transcriptase control; NT, no template for reverse transcription control; H₂O, no template for PCR control.