FIGURE 8.

Mfap5 mRNA is expressed by cells of the aorta, mitral valve, coronary vessels, kidney, lung, and pinnae. In situ hybridization to detect Mfap5 (A and D–I), Mfap2 (B), and Col1a1 (C), expression was performed on 14-μm sections from 4-month-old wild-type mice. A and A′, arrows demonstrate digoxigenin-labeled mRNA (blue) localized to the intima (arrow) and adventitia (arrowhead) of the aorta. A′ is a magnification of the boxed region in A. B, Mfap2 mRNA is expressed throughout the aorta (arrow). C, Col1a1 mRNA is heavily expressed in the aortic adventitia (arrow). D and D′, Mfap5 is expressed in the atrialis of the mitral valve (MV, arrow). E, Mfap5 mRNA is found in the coronary vessel (CV, arrow). F, significant Mfap5 expression is localized to the aortic root (AR, arrow). G and G′, large airways and arterioles (G′, arrow) are positive for Mfap5 expression. H, large vessels of the kidney express Mfap5 (arrow). J, J′, and K, no nonspecific reactivity is detected in Mfap5 sense control sections. Scale bar = 300 μm. Micrographs were taken using identical camera settings and image processing.