FIGURE 2.

miR-433 inhibits HCC cell migration. A, Transwell cell migration assay. MHCC97H cells were transfected with pTarget (control; 2 μg), pTarget-miR-433 (miR-433; 2 μg), anti-miR negative control (negative; 20 nm), or miR-433 inhibitor (anti-miR-433; 20 nm). Migration assays were performed, and migrated cells were counted. *, p < 0.01. B, qPCR analysis of miR-433 levels. *, p < 0.05. C, Wound healing assay. MHCC97H cells were transfected with anti-miR negative control (20 nm) or miR-433 inhibitor (20 nm). Images were taken at 0, 8, and 16 h post-scratch. Red lines indicate the wound gap. D, focus formation assay. HepG2 cells were transfected with the pTarget or pTarget-miR-433 (miR-433) plasmid (2 μg) and seeded in 0.4% top agar onto 6-well plates coated with 0.7% bottom agar. Ten days later, colonies were counted from triplicate plates, and one representative plate is shown. Statistical results represent the mean ± S.D. of focus counts. *, p < 0.01, pTarget-miR-433 versus pTarget. Error bars represent S.E.