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Effect of TSA and VPA on GR-hsp90 interactions. Hepa-1c1c7 cells (A and B) or 1470.2 cells (C) were treated with TSA (200 nm) (A and C) or VPA (5 mm) (B) for up to 5 h. Cytosolic extracts were generated. Overall levels of GR and hsp90 were measured by Western blot. GR-hsp90 interaction was measured by immunoprecipitation (IP) with GR or GFP antibody (Ab) followed by Western blotting with antibodies to GR or hsp90. The results are representative of two to three independent experiments.
