TABLE 1.
N-terminal sequences determined by Edman sequencing
| Protein | N-terminal sequence |
|---|---|
| AccA | S2LNFLDa |
| AccD | (L/F)K16ASIPb |
| AphA | L24ASSPS |
| DegP | F84FGDDSP |
| Dps | L6VKSK |
| FtsY | F8FSWLGFG |
| GyrB | (L/F)R394KGALDL |
| IciA | L69LRQVELL |
| InfB | (L/F)K87KRTFVc |
| (L/F)K88RTFVKRc | |
| InfB-AZ | (L/F)K64TRSTLN |
| LacI | (L/F)K33TREKV |
| L56AGKQS | |
| MreB | F103MRPSP3 |
| MreB-AZ | F94IKQVHS |
| (L/F)K96QVHSNS | |
| NusG | F102IGGTS |
| Odo1/SucA | (L/F)R68LAKDAS |
| OsmE | F31VQPVVKD |
| Pat | (F/L/L/L)M1NRLPS |
| RpsA | F130LPGSLVD |
| RsgA | (L/F)R21LKTS |
| Tig | (L/F)K46GKVP |
| TufA/B (EF-Tu) | (L/F)K5FERTK |
| UspG | M1YKTIIMP |
| YbaB | (L/F)K4GGLGNL |
| YgiC | L182TGTLAG |
| YpfJ | L57MTGQPVS |
| YtfR | F402LSGGNNQQ |
a The superscript represents the amino acid position in the open reading frame encoded by the gene for that protein.
b Amino acids in parentheses are leucine or phenylalanine residues added by Aat.
c These N-terminal sequences were obtained for truncated forms of the native protein pulled down from wild-type cells and for the cleavage products of the corresponding AZ fusion proteins.