TABLE 3.
Proteins significantly enriched in lysates from Aat+ cells compared with Aat− cells
Cells were grown in LB to stationary phase (≤16 h). Proteins were judged to be Aat substrates if they were present in ClpS pulldowns from wild-type cells but were absent in the pulldowns from aat mutant cells. Of the 17 proteins selected by this criterion, 13 had been independently identified as Aat substrates based on N-terminal sequencing.
| Protein ID | Spectral count |
G value | p value | N-terminal sequencea | |
|---|---|---|---|---|---|
| Aat+ | Aat− | ||||
| AccA | 8 | 0 | 6.2 | 1.3 × 10−2 | S2LNFLD |
| AccD | 5 | 0 | 3 | 8.8 × 10−2 | (L)K16ASIP |
| AldB | 11 | 0 | 9.8 | 1.8 × 10−3 | |
| AtpA | 11 | 0 | 9.8 | 1.8 × 10−3 | M1QLNSTEISELIKb |
| AtpD | 11 | 0 | 9.8 | 1.8 × 10−3 | |
| FhuF | 6 | 0 | 3.9 | 4.6 × 10−2 | |
| GrpE | 7 | 0 | 5 | 2.5 × 10−2 | |
| GyrB | 12 | 0 | 11 | 9.2 × 10−4 | (L)R394KGALDL |
| InfB | 32 | 0 | 37 | 1.3 × 10−9 | (L)K87KRTFV |
| (L)K88RTFVKR | |||||
| Odo1/SucA | 10 | 0 | 8.5 | 3.5 × 10−3 | (L)R68LAKDAS |
| PatA (PATase) | 20 | 0 | 21 | 4.4 × 10−6 | (LLL)M1NRLPS |
| RecT | 7 | 0 | 5 | 2.5 × 10−2 | |
| RsgA | 9 | 0 | 7.3 | 6.7 × 10−3 | (L)R21LKTS |
| Tig | 25 | 0 | 28 | 1.5 × 10−7 | (L)K46GKVP |
| TufA/TufB (EF-Tu) | 18 | 0 | 19 | 1.7 × 10−5 | (L)K5FERTK |
| YbaB | 9 | 0 | 7.3 | 6.7 × 10−3 | (L)K4GGLGNL |
| YggL | 12 | 0 | 11 | 9.2 × 10−4 | |
a N-terminal sequences were determined by Edman degradation with the exception of AtpA. The superscript indicates the position of the amino acid in the deduced open reading frame encoded by the corresponding gene. The (L) indicates a leucine residue added to the N terminus by Aat.
b N-terminal peptide for AtpA was determined by MS/MS.