FIGURE 1.

A, α-Tm E180G/S283A DMTG construct. The α-MHC promoter drives cardiac-specific expression of the striated muscle α-Tm with encoded substitutions at amino acids (aa) 180 (E180G) and 283 (S283A) on the same molecule. B, myofibrillar preparations resolved by SDS-PAGE and stained with Coomassie Blue. Note the small shift in migration between the α-Tm E180G and DMTG Tm samples. Arrows indicate DMTG endogenous and TG protein. C, quantification of endogenous Tm protein remaining in NTG, α-Tm E180G, α-Tm S283A, and DMTG samples. Error bars represent S.E.