A, Western blots of sarcoplasmic Ca2+ flux proteins in NTG, α-Tm E180G, α-Tm S283A, and DMTG hearts. B, quantification of phosphorylation levels of PLN Ser-16 and PLN Thr-17. C, quantification of expression of PLN and SERCA2a. D, Western blots of TnI and phosphorylated TnI (pTnI) from NTG, α-Tm E180G, α-Tm S283A, and DMTG hearts. E, quantification of data from D. *, versus NTG, p < 0.05; #, versus α-Tm E180G, p < 0.05; @, versus α-Tm S283A, p < 0.05; n = 8. Error bars represent S.E.