FIGURE 2.

FGF signaling in DESCs is required to prevent differentiation. A, phase contrast images of DESC sphere untreated or treated with 1 μm FGFR inhibitors as indicated. B, H&E-stained section of paraffin-embedded DESC spheres cultured in the presence or absence of 1 μm FGFR inhibitors as indicated, showing the sphere morphology. C, real-time RT-PCR analyses of amelogenin (Amg) and CK14 in DESC spheres with or without FGFR inhibitor treatment. D, sections of paraffin-embedded DESC spheres were immunostained with antibodies against amelogenin or CK14. Data were normalized to β-actin housekeeping genes with DMSO groups as 1. FRi, FGFR inhibitor 341608. *, p < 0.05. TP3, To-PRO-3 staining; FRi, FGFR inhibitor 341608.