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. 2013 Aug 26;288(40):28952–28961. doi: 10.1074/jbc.M113.506873

FIGURE 6.

FIGURE 6.

FGF signaling is required for the Wnt pathway to up-regulate Lgr5 expression. A, whole-mount LacZ staining of teeth cultured in the presence or absence of Wnt3a, LiCl, or FGFR inhibitor (FRi) as indicated. a–f′ are sections of the same tissues shown in a–f. B, total RNA was extracted from the CL regions cultured in the presence or absence of Wnt3a, LiCl, or FGFR inhibitor as indicated for RT-PCR analyses of endogenous Lgr5 expression. C, whole-mount LacZ staining of teeth cultured in the presence or absence of FGF1 or FGF2 as indicated. a–c′ are sections of the same tissues shown in a–c. The areas and intensities of LacZ staining in all sections were quantitated by NIH ImageJ. The data were normalized to the untreated group and expressed as -fold increase. Numbers in a–c′ are means ± S.D. of two replicated samples. D, whole-mount LacZ staining of teeth bearing the Axin2LacZ reporter allele cultured in the presence of control (L, L-cell) or Wnt3a-conditioned medium. b′ and d′ are sections of the same tissue shown in b and d. Arrowheads indicate the LacZ+ stromal tissues. Representative data from three independent experiments are shown. E, total RNA was extracted from the CL regions cultured in the presence or absence of Wnt3a or FGFR inhibitor as indicated for RT-PCR analyses of axin2 expression. The dotted lines outline the CLs. *, p < 0.05. Data are means ± S.D. of triplicate samples.