Nonfunctional Orai1 N-truncation mutants introduced into the Orai1 V102A core display no shift in reversal potential in the presence of STIM1.
a, I/V relationships of normalized Orai1 V102A currents in comparison with those of Orai1 ΔN1–75 V102A, Orai1 ΔN1–76 V102A, and Orai1 ΔN1–78 V102A. b, analogue to panel a in the presence of STIM1. c, block diagram displaying reversal potentials of Orai1 V102A, Orai1 ΔN1–75 V102A, Orai1 ΔN1–76 V102A, and Orai1 ΔN1–78 V102A currents in the absence and presence of STIM1 (*, p < 0.05). Error bars indicate S.E. n.s., not significant.