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The interactions between the OB4 domains of Cdc13A and Cdc13B. A, the fusion proteins used in the co-expression and GST affinity purification analyses are illustrated. B, the total proteins and the levels of bait and target proteins in the cell extracts were analyzed by SDS-PAGE followed by Coomassie staining and anti-FLAG Western, respectively. C, the levels of the bait and target proteins in the GST affinity elution fractions were analyzed by SDS-PAGE and Coomassie staining.
