TABLE 1.
Cleavage rates
The rates are described as kcl values, fitted from the slope of the reaction velocities from two independent experiments. The -fold reduction is the reduction of the rate relative to that of the pseudo-WT with the same substrate. NA is data not available.
| Mutant | k3′O (×10−3) | k3′S (×10−3) | k3′S/k3′O | k3′O | k3′S |
|---|---|---|---|---|---|
| % cleaved/s | % cleaved/s | % cleaved/s | -fold reduction | -fold reduction | |
| Pseudo-WT | 420 ± 22 | 281 ± 2.8 | 0.67 | ≡1 | ≡1 |
| R7A | ∼0 | ∼0 | NA | NA | NA |
| R7K | ∼0 | ∼0 | NA | NA | NA |
| R43A | 18.8 ± 0.84 | 1.86 ± 0.16 | 0.099 | 22.4 | 151 |
| R43K | 53.1 ± 2.7 | 1.02 ± 0.65 | 0.019 | 7.9 | 275 |
| R66A | ∼0 | ∼0 | NA | NA | NA |
| R66K | ∼0 | ∼0 | NA | NA | NA |
| R69A | 0.0518 ± 0.0033 | 0.490 ± 0.025 | 9.5 | 8110 | 573 |
| R69K | 1.52 ± 0.072 | 2.22 ± 0.044 | 1.5 | 276 | 127 |
| R125A | 187 ± 5.3 | 103 ± 2.8 | 0.56 | 2.25 | 2.73 |
| R125K | 170 ± 4.8 | 39.0 ± 1.7 | 0.23 | 2.47 | 7.21 |
| R131A | 85.5 ± 2.7 | 55.2 ± 1.2 | 0.65 | 4.91 | 5.09 |
| R131K | 206 ± 7.8 | 87.3 ± 2.6 | 0.42 | 2.04 | 3.22 |