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. 2013 Jul 30;14(9):772–779. doi: 10.1038/embor.2013.108

Figure 2.

Figure 2

RIG-I binds hairpins with one triphosphate with a 1:1 stoichiometry. Hydrodynamic analysis of RIG-I in complex with 5′ppp10L, 5′ppp20L, 5′ppp30L and 5′pppGC22 RNA. c(s) distributions for each SV experiment were plotted against the sedimentation coefficient (s20,w). Peak s20,w values for each distribution are 6.0 for RIG-I alone and 6.2, 6.4, 6.9 and 9.3 for RIG-I: 5′ppp10L, 5′ppp20L, 5′ppp30L and 5′pppGC22 complexes, respectively. Estimated molecular weights from Sedfit are 106 kDa (f/f0=1.31), 113 kDa (f/f0=1.31), 121 kDa (f/f0=1.53), 133 kDa (f/f0=1.57) and 228 kDa (f/f0=1.45), respectively. Models of RIG-I bound to each RNA construct are shown next to each c(s) distribution. RIG-I, retinoic acid-inducible gene-I; SV, sedimentation velocity.