Figure 3.

CK2 activity is not essential for bulk macroautophagy and pexophagy. (A,B) WT (TKYM236) and CK2ts mutant (TKYM261) strains were grown in YPD medium and shifted to SD-N and cultured at 37 °C for 3 or 6 h (A). WT (TKYM236) and atg1Δ (TKYM256) strains were grown in YPD medium and shifted to SD-N with or without 70 μM TBB supplementation and cultured for 0, 3 or 5 h (B). Samples were collected and protein extracts assayed for Pho8Δ60 activity. The results represent the mean and standard deviation of three experiments. (C) WT and atg1Δ cells expressing Pex14-GFP were cultured with oleic acid containing medium for 19 h, and then shifted to SD-N with or without 70 μM TBB supplementation, cultured for 0, 2 or 4 h, and monitored for GFP processing by immunoblotting. CK2, Casein kinase 2; GFP, green fluorescent protein; TBB, 4,5,6,7-tetrabromobenzotriazole; WT, wild type; YPD, yeast extract-peptone-glucose.

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