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Clustal omega alignment of the amino-terminal region of LegAS4 and RomA in Legionella pneumophila Lp02 and Paris, respectively. Highlighted in yellow are the 13 N-terminal amino acids found in LegAS4 but missing in RomA. Highlighted in green is the NLS region, which shows three amino acid differences between LegAS4 and RomA, and highlighted in blue is an eight amino acid stretch that shows poor sequence homology. NLS, nuclear localization signal.
