Figure 1.

Parent-of-origin effects on the transmission of the active EVD within inter-accession crosses. (A) Crossing scheme to obtain epi12 and its F3 cross to the Ler accession. One plant from line 12 (epi12) of the epiRIL population at the F₃ generation was crossed reciprocally (double arrow) with Ler to generate F₁ and reciprocal F₁ (rF₁) hybrids. The use of Ler (L) or the epiRIL (e) parent is indicated (maternal/paternal) for each hybrid. The inferred EVD activity levels (EVD-silent or EVD-active) are indicated by white and black circles, respectively. (B) Southern blot detection of EVD ecDNA reveals pollen transmission of EVD. Undigested genomic DNA was probed for EVD. The presence of the ∼5-kb EVD ecDNA is indicated (black arrow). Individual epiRIL siblings were investigated, while the two F₁ samples and the Ler parental control each comprised a bulked lot of eight individual plants. ecDNA, extrachromosomal DNA; Ler, Landsberg erecta.