TABLE 1.

Summary of the whole-genome sequence information of five oyster-associated bacterial isolates having the ability to degrade crude oil hydrocarbons

Isolate	Isolation sourcea	GenBank accession no.	No. of contigs	Length of assembly (bp)	N50 (bp)	GC content (%)	Total no. of putative genes	Total no. of putative genes for xenobiotic degradation and metabolism
P. stutzeri MF28	Mantle fluid	ATAR00000000	91	4,943,564	128,670	62.29	4,630	813
P. alcaligenes OT69	Oyster tissue	ATCP00000000	223	7,029,758	96,981	65.96	6,543	976
P. aeruginosa WC55	Water column	ATAQ00000000	342	6,844,176	92,012	66.14	6,650	958
S. maltophilia MF89	Mantle fluid	ATAP00000000	209	4,649,035	69,908	66.18	4,376	619
M. maritypicum MF109	Mantle fluid	ATAO00000000	260	3,996,920	158,001	68.24	4,094	608

^aOysters from Apalachicola Bay, FL, were collected using a tong. The sampling site (Dry Bar, 29°40.425'N, 85°03.406'W) is located within the Apalachicola river’s hydrologic discharge channel just southwest of the river mouth (11) and is the most productive oyster harvesting area in Florida. Oysters were culled on the boat, and 20 adult oysters of approximately the same size were collected. Additionally, 1 liter of overlaying water from the oyster reef was collected in a sterile bottle. Samples were stored on ice and transported to Florida A&M University for further processing. In the laboratory, oysters were rinsed using sterile 0.85% NaCl to remove debris and shell biofilm and each oyster was carefully shucked using sterile knives. Prior to collection of the oyster tissues, mantle fluid from the oysters was collected using sterile syringes. The details of further processing of these samples to isolate the oil-degrading microorganisms will be reported in a forthcoming manuscript.