Fig. 4.

Assessment of the intensity of protein S-nitrosylation in 2-cell mouse embryos using the biotin switch method. a The NO donor (SNP) significantly increased protein S-nitrosylation, whereas the NOS inhibitor (LNA) did not significantly reduce S-nitrosylation. The addition of the cGMP mimic BrGMP and the GC inhibitor ODQ had nonsignificant effects on S-nitrosylation. b Addition of LNA or GSHM to NO-exposed embryos reduced S-nitrosylation significantly. However, TBAP supplementation alone or SNP+TBAP induced an increase in protein S-nitrosylation. * p < 0.05 and **p < 0.01 compared to the control group according to Student’s t-test. The vertical bars and bars of error represent the mean and standard error of at least 3 different experiments, respectively. Each group contained at least ten 2-cell embryos