Table 2.
The effect of morula and early blastocyst cryopreservation on day 5 blastocyst cell number and allocation. Data expressed as mean ± SEM. n ≥ 19 embryos per treatment (3 replicate experiments) a–d: Different superscripts within a column are significantly different (P < 0.01) ICM: inner cell mass, TE: trophectoderm CM: compact morula, EB: early blastocyst
| Treatment | Total | ICM | TE | % ICM/total |
|---|---|---|---|---|
| Control | 73.9 ± 2.8a | 16.1 ± 0.7a,b | 54.9 ± 2.2 | 23.1 ± 0.7a,c,d |
| Vitrification CM | 61.3 ± 2.2a,b | 16.2 ± 0.4a | 45.0 ± 2.0 | 27.0 ± 0.8b |
| Slow freezing CM | 60.6 ± 2.9a,b | 15.4 ± 0.7a,b | 45.2 ± 2.4 | 25.7 ± 0.8a,b,c |
| Vitrification EB | 61.7 ± 3.6a,b | 13.3 ± 1.1b,c | 48.4 ± 2.8 | 21.6 ± 1.1c,d |
| Slow freezing EB | 57.4 ± 3.2b | 11.8 ± 0.8c | 45.6 ± 2.7 | 20.8 ± 0.9d |